Anti- Chlamydia Pneumoniae Antibody (Cpn-IgM) ELISA Kit
Reactivity: Chlamydophila pneumoniae
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- Target
- Anti- Chlamydia Pneumoniae Antibody (Cpn-IgM)
- Reactivity
- Chlamydophila pneumoniae
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of Chlamydia pneumoniae IgM in the research laboratory
Alternative Names: Chlamydia pneumoniae IgM ELISA kit
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- Application Notes
- Optimal conditions to be determined by end user
- Assay Procedure
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Diluted patient serum (serum diluent contains sorbent to remove rheumatoid factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibodyantigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Anti- Chlamydia Pneumoniae Antibody (Cpn-IgM)
- Target Type
- Antibody
- Background
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Chlamydia pnemoniaee, the third recognized of five possible species of Chlamydia (trachomatis, psittaci, pneumoniae, pecorum and an as-yet-unnamed species) was formerly known as Chlamydia spp. strain TWAR. This respiratory pathogen which causes acute respiratory disease, pnemoniae and pharyngitis is often isolated from patients with otitis media with effusion, pnemoniae with pleural effusion and in asymptomatic respiratory tract infections. C. pnemoniae causes up to 10 % of community-acquired pnemoniae cases and it is also a risk factor for coronary heart disease and Guillain-Barré syndrome. Seroprevalence of C. pnemoniae among children is low and increases sharply in teenagers, continues to increase until middle age, and remains high (>50 %) into old age, suggesting that most people have more than one C. pnemoniae infection during their lifetime. Primary chlamydial infection is characterized by a predominant IgM response within 2 to 4 weeks and a delayed IgG and IgA response within 6 to 8 weeks. After acute C. pnemoniae infection, IgM antibodies are usually lost within 2 to 6 months IgG antibody titers rise and usually decrease slowly, whereas IgA antibodies tend to disappear rapidly. When primary chlamydia infection is suspected, the detection of IgM is highly diagnostic. In reinfection, IgM level may be rarely detected while IgG and IgA levels rise quickly, often in one to two weeks. IgA antibodies have shown to be a reliable immunological marker of primary, chronic and recurrent infections. These antibodies usually decline rapidly to baseline levels following treatment and eradication of the chlamydia infections.
Synonyms: Chlamydia pneumoniae IgM ELISA kit.
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