Pregnenolone ELISA Kit

Catalog No. ABIN930535

Product Details

Target: Pregnenolone

Reactivity: Hormone

Method Type: Competition ELISA

Application: ELISA

Characteristics: ELISA kit for the detection of Pregnenolone in the research laboratory
Alternative Names: Pregnenolone ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competitionoccurs between an unlabeled antigen (present in standards, control and patient samples) and an enzymelabelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. Theintensity of the colour formed is inversely proportional to the concentration of pregnenolone in the sample. A set ofstandards is used to plot a standard curve from which the amount of pregnenolone in patient samples and controls canbe directly read.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: Pregnenolone

Target Type: Hormone

Background: Pregnenolone (3-beta-hydroxypregn-5-en-20-one) is the first steroid to be derived from cholesterol in the pathway of steroidogenesis, and it is the common precursor for all of the adrenal and gonadal steroids. Its production occurs in the mitochondrion by cleavage of the C20 side chain of cholesterol by the P450SCC enzyme. Once produced, pregnenolone may be utilized by two pathways of steroidogenesis. Pregnenolone may either be converted to 17-OH pregnenolone via the enzymatic action of 17-alphahydroxylase or to progesterone via the enzymatic action of 3-beta-hydroxysteroid dehydrogenase. Elevated pregnenolone levels occur in forms of congenital adrenal hyperplasia (CAH), due to 3-beta-hydroxysteroid dehydrogenase deficiency or 17-alpha-hydroxylase deficiencies. Higher levels have also been reported in women with idiopathic hirsutism. Studies on pregnenolone levels in regard to sex and age differences indicate that maximum levels occur at approximately 17 and 16 years of age for women and men, while minimum levels occur at approximately 37 and 38 years of age for women and men, respectively. In general, women were found to have slightly higher values when compared to men.
Synonyms: Pregnenolone ELISA kit.