ANCA ELISA Kit
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- Target
- ANCA
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Characteristics
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ELISA kit for the detection of ANCA combi in the research laboratory
Alternative Names: Anti-neutrophil cytoplasmic antibodies ELISA kit, ANCA ELISA kit
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- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
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PR3, MPO, BPI, Elastase, Cathepsin G, Lysozyme and Lactoferrin are bound separatedly to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color.The addition of an acid stops the reaction forming a yellow endproduct.The intensity of this yellow color is measured photometrically at 450 nm.The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- ANCA
- Target Type
- Disease
- Background
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Recently, the main antigens for the c and pANCAs have been identified. The target antigen for 80-90 % of cANCA antibodies is the proteinase 3 (PR3), a serine proteinase from agranules. 10-20 % of cANCAs are directed to other proteins. The solubilization of ethanol-fixed granulocytes causes a binding of positively charged proteins around the nucleus. Antibodies to these proteins appear in the immunofluorescence as pANCAs. Approximately 90 % of pANCA positive sera contain autoantibodies directed to myeloperoxidase (MPO) which is located in the granula of neutrophilic granulocytes. Antibodies to other antigens e.g. lactoferrin, elastase, cathepsin G and lysozyme often result in similar pANCA patterns. Beside different untypical variants of pANCA, IF patterns of granulocyte specific antinuclear antibodies (GSANA) are indistinguishable from those of pANCAs. A distinct interpretation and classification of the IF patterns is quite difficult. Therefore every positive IFANCA finding especially pANCAs should be differentiated by ELISA techniques using purified antigens. The antiPR3 antibody titer correlates well with the clinical status of the disease. Antibody titers are decreasing under therapy and become negative after remission. Anti-MPO levels correlate with the clinical status too. They are always higher during the active disease than after remission.
Synonyms: Anti-neutrophil cytoplasmic antibodies ELISA kit, ANCA ELISA kit.
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