Anti-Phosphatidic Acid IgG/IgM ELISA Kit
Reactivity: Chemical
Colorimetric
Sandwich ELISA
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- Target
- Anti-Phosphatidic Acid IgG/IgM
- Reactivity
- Chemical
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Characteristics
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ELISA kit for the detection of Phosphatidic Acid IgG/IgM in the research laboratory
Alternative Names: Phosphatidic Acid IgG/IgM ELISA kit
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- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
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AntiPhosphatidic Acid is an indirect solid phase enzyme immunometric assays (ELISA). It is designed for the quantitative measurement of IgG or IgM class autoantibodies directed against negativelycharged phospholipids. The microplate is coated with highly purified Phosphatidic Acid. AntiPhospholipid autoantibodies require beta2Glycoprotein I as a cofactor for binding. The microplate is therefore saturated with human beta2Glycoprotein I. The microplates can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module (
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Anti-Phosphatidic Acid IgG/IgM
- Sub Type
- Fusionprotein
- Target Type
- Antibody
- Background
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Under the acronym aPL (antiPhospholipid antibodies) antibodies against negatively-charged phospholipids, such as CL (Cardiolipin), LA (Lupus Anticoagulant), PS (Phosphatidyl Serine), PI (Phosphatidyl Inositol) and PA are summarised. Of these, Cardiolipin is the phospholipid most commonly used as antigen to test for aPL by ELISA. Some antisera which bind cardiolipin-coated ELISA plates can also bind to plates coated with other negatively-charged phospholipids, such as Phosphatidyl Serine (PS), Phosphatidyl Inositol and Phosphatidic Acid (PA). Some investigators have suggested that the use of PS in place of cardiolipin in ELISA tests enables more specific diagnosis. These antigens are less commonly used and their additional use can improve the clinical sensitivity in patient samples with suspected APS (Anti Phospholipid Syndrome), but they can't replace the measurement of autoantibodies against Cardiolipin.
Synonyms: Phosphatidic Acid IgG/IgM ELISA kit.
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