Centromere B ELISA Kit

Catalog No. ABIN930751

Various Species ELISA Kit Colorimetric assay for quantification of Various Species .

Quick Overview for Centromere B ELISA Kit (ABIN930751)

Target: Centromere B

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Centromere B in the research laboratory
Alternative Names: Centromere B ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

AntiCentromer B is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against Centromer B. The microplates can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module (

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: Centromere B

Target Type: DNA

Background: In patients suffering from CREST syndrome circulating antibodies against the centromere protein B can be detected. Up to 70 % of all CREST patients exhibit anti-centromere B autoantibodies. The determination of anti-centromere B autoantibodies is of prognostic significance in diagnosing Raynaud's phenomenon. Raynaud's phenomenon often comes out as the first symptom of scleroderma and precedes the additional manifestations by several years. Also in patients suffering from primary biliary cirrhosis (PBC) anti-centromere B antibodies can be found. PBC and CREST often overlap. Anti-centromere B are found in about 10-20 % of sera from patients with PBC preferentially identifying those patients with Raynaud's phenomenon and sclerodactyly. Along with other centromere proteins (e.g. CENPA (19 kDa), CENPC (140 kDa)) centromere protein B (80 kDa) belongs to a protein complex of the chromosomal kinetochore, the attachment site of microtuble spindles during cell division.
Synonyms: Centromere B ELISA kit.