Epitope Tag Protein Marker Lysate

Details for Product No. ABIN925598, Supplier: Log in to see
Application
Western Blotting (WB)
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Specificity Liquid (in 1x Loading Buffer)
Lysate Fraction Whole Cell Lysate
Lysate Type Overexpression Lysate
Background This product is intended as a positive control for use with anti-epitope tag antibodies.  Epitope tags are short peptide sequences easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
Synonyms: Flag, MBP, HA, 6XHIS, Lysate
Application Notes This product is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer, heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for western blotting. The product is supplied in 1X loading buffer and is ready-to-use. 62.5 mM Tris HCl, 10% Glycerol, 2% SDS, 0.05% bromphenol blue, pH 6.8 + 1% Triton 100 + 2.5 mM EDTA+5mM Tris HCl+4% (v/v) β-Mercaptoethanol.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
Western Blotting (WB) image for Epitope Tag Protein Marker Lysate (ABIN925598) Western blot of 12-Epitope Tag Protein Marker lysate. The blot shows intense signal c...