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- Application
- Western Blotting (WB)
- Specificity
- Liquid (in 1x Loading Buffer)
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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BIRC3 293T Cell Transient Overexpression Lysate(Denatured)
BIRC3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
APLP2 293T Cell Transient Overexpression Lysate(Denatured)APLP2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
BAG1 293T Cell Transient Overexpression Lysate(Denatured)BAG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
VNN1 293T Cell Transient Overexpression Lysate(Denatured)VNN1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)CYP26A1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
Hep2 Whole Cell LysateWB Whole Cell Lysate Cell Lysate HepG2 Cells Reactivity: Human
NIH/3T3 Whole Cell Lysate (PDGF Stimulated)WB Whole Cell Lysate Cell Lysate 3T3 Cells PDGF Stimulated Reactivity: Mouse
DYKDDDDK Positive Control LysatePC, WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Tag
A431 Whole Cell Lysate (EGF Stimulated)WB Whole Cell Lysate Cell Lysate A431 Cells EGF treated Reactivity: Human
CD177 (human): 293T LysateCD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
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- Application Notes
- This product is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer, heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for western blotting. The product is supplied in 1X loading buffer and is ready-to-use. 62.5 mM Tris HCl, 10% Glycerol, 2% SDS, 0.05% bromphenol blue, pH 6.8 + 1% Triton 100 + 2.5 mM EDTA+5mM Tris HCl+4% (v/v) β-Mercaptoethanol.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.
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- Background
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This product is intended as a positive control for use with anti-epitope tag antibodies. Epitope tags are short peptide sequences easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
Synonyms: Flag, MBP, HA, 6XHIS, Lysate
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