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Our data revealed that both miR-199a-3p and its target gene AK4 are reversely correlated with the osteosarcoma drug resistance.
Results found that AK4 was involved in hypoxia tolerance, resistance to anti-tumor drug, and the regulation of mitochondrial activity.
a stress-protective function of AK4
Data indicate that patients with high adenylate kinase-4 (AK4) and low transcription factor ATF3 expression showed unfavorable outcomes compared with patients with low AK4 and high ATF3 expression.
The AK4 cDNA was expressed in Escherichia coli and the substrate specificity and kinetic properties of the recombinant protein were characterized.
To investigate the role of hinge IV, crystal structure of human adenylate kinase 4 (AK4) L171P mutant was determined.
This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.
, GTP:AMP phosphotransferase AK4, mitochondrial
, adenylate kinase 3-like 1
, adenylate kinase isoenzyme 4, mitochondrial
, mitochondrial adenylate kinase-3
, nucleoside-triphosphate-adenylate kinase
, Adenylate kinase isoenzyme 4, mitochondrial
, adenylate kinase 3
, adenylate kinase 3-like protein 1
, adenylate kinase 3 alpha-like 1
, adenylate kinase 3-like 2
, adenylate kinase 4