DYKDDDDK Tag peptide (DYKDDDDK Tag)

Details for Product No. ABIN1607595, Supplier: Log in to see
Protein Name
Origin
Tag
1
Purification tag / Conjugate
DYKDDDDK Tag
Application
Control Peptide (CP), Immunoprecipitation (IP), Western Blotting (WB)
Options
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Purity > 90 % specific peptide by HPLC and MS.
Endotoxin Level Low Endotoxin : No
Protein Name
Target Type Tag
Background The DYKDDDDK peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free DYKDDDDK Peptide. Synonyms: synthetic DYKDDDDK peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide
Application Notes A working concentration of 500 μg/mL for triple DYKDDDDK peptide is typically used for competitive elution of DYKDDDDK containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK DYKDDDDK affinity gel PROTOCOL for specific instructions for use.
Restrictions For Research Use only
Format Lyophilized
Reconstitution 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Concentration 1 mg/mL
Storage 4 °C/-20 °C
Storage Comment Store vial at 2 - 8 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Expiry Date Expiration date is six (6) months from date of opening.
Supplier Images
Western Blotting (WB) image for DYKDDDDK Tag peptide (DYKDDDDK Tag) (ABIN1607595) SDS-PAGE of Anti-DYKDDDDK Affinity Gel. Lane 1: Cell lysate before purification. Lan...
Western Blotting (WB) image for DYKDDDDK Tag peptide (DYKDDDDK Tag) (ABIN1607595) SDS-PAGE of Anti-DYKDDDDK Affinity Gel. Lane 1: crude lysate containing over-express...
SDS-PAGE (SDS) image for DYKDDDDK Tag peptide (DYKDDDDK Tag) (ABIN1607595) SDS-PAGE of Anti-DYKDDDDK affinity gel using DYKDDDDK control peptide. Lane 1: DYKDDD...