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- Target See all DYKDDDDK Tag products
- DYKDDDDK Tag
- Origin
- Tag
- Application
- Control Peptide (CP), Immunoprecipitation (IP), Western Blotting (WB)
- Purity
- > 90 % specific peptide by HPLC and MS.
- Endotoxin Level
- Low Endotoxin : No
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DYKDDDDK Tag peptide (DYKDDDDK Tag)
Reactivity: Tag CP, IP, WB
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- Application Notes
- A working concentration of 500 μg/mL for triple DYKDDDDK peptide is typically used for competitive elution of DYKDDDDK containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK DYKDDDDK affinity gel PROTOCOL for recommendations for use.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
- Concentration
- 1 mg/mL
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2 - 8 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- Expiration date is six (6) months from date of opening.
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- Target
- DYKDDDDK Tag
- Target Type
- Tag
- Background
- The Triple DYKDDDDK Peptide is a synthetic peptide of 23 amino acid residues (molecular weight of 2861.9 daltons). The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic DYKDDDDK sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK). The triple DYKDDDDK peptide is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free triple DYKDDDDK Peptide. Synonyms: synthetic DYKDDDDK triple peptide, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide
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