Fas Ligand Inhibitor Peptide
Reactivity: Human, Mouse, Rat
Func
Catalog No. ABIN925004
Quick Overview for Fas Ligand Inhibitor Peptide (ABIN925004)
Target
Fas Ligand Inhibitor
Origin
Human, Mouse, Rat
Application
Functional Studies (Func)
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Specificity
- Inhibits the apoptosis-inducing activity of Fas Ligand. Binds FasL (APO-1L, CD95L, CD178).
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Characteristics
- Extracellular domain of human Fas (aa. 7-154) fused to Fc portion of human IgG1.
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Purity
- 95 % as determined by SDS-PAGE
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Endotoxin Level
- <0.1 EU/µg purified protein as determined by LAL test.
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Application Notes
- Functional Application: Inhibition of FAS Ligand activity in human and mouse: The apoptosis-inducing activity of FasL is inhibited by concentrations of 20-100 µg/mL of the fusion protein. Concentrations of Fas Ligand Inhibitor required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with 50 µL sterile water to produce a 1 mg/mL solution in PBS. Further dilutions should be made with medium containing 5% fetal calf serum (FCS) or a carrier protein.
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Buffer
- PBS
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Storage
- -20 °C
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- Fas Ligand Inhibitor
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Background
- Fas is a 45 kDa type I transmembrane protein belonging to the TNF receptor family. Fas contains 3 cysteine-rich repeats in the extracellular portion and a cytoplasmic motif called the `death domain' (DD). Death signals are triggered upon binding to its trimeric ligand FasL which induces oligomerization of Fas. The DD associates with FADD, which in turn connects to aspartate-specific proteases (caspases) that are implicated as mediators of most types of apoptotic cell death. Synonyms: APO-1, CD95, TNFRSF 6
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Molecular Weight
- 45 kDa
Target
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