Fas Ligand Inhibitor Peptide
Reactivity: Human, Mouse, Rat
Func
-
- Target
- Fas Ligand Inhibitor
- Origin
- Human, Mouse, Rat
- Application
- Functional Studies (Func)
- Specificity
- Inhibits the apoptosis-inducing activity of Fas Ligand. Binds FasL (APO-1L, CD95L, CD178).
- Characteristics
- Extracellular domain of human Fas (aa. 7-154) fused to Fc portion of human IgG1.
- Purity
- 95 % as determined by SDS-PAGE
- Endotoxin Level
- <0.1 EU/µg purified protein as determined by LAL test.
-
- Application Notes
- Functional Application: Inhibition of FAS Ligand activity in human and mouse: The apoptosis-inducing activity of FasL is inhibited by concentrations of 20-100 µg/mL of the fusion protein. Concentrations of Fas Ligand Inhibitor required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute with 50 µL sterile water to produce a 1 mg/mL solution in PBS. Further dilutions should be made with medium containing 5% fetal calf serum (FCS) or a carrier protein.
- Buffer
- PBS
- Storage
- -20 °C
-
- Target
- Fas Ligand Inhibitor
- Background
- Fas is a 45 kDa type I transmembrane protein belonging to the TNF receptor family. Fas contains 3 cysteine-rich repeats in the extracellular portion and a cytoplasmic motif called the `death domain' (DD). Death signals are triggered upon binding to its trimeric ligand FasL which induces oligomerization of Fas. The DD associates with FADD, which in turn connects to aspartate-specific proteases (caspases) that are implicated as mediators of most types of apoptotic cell death. Synonyms: APO-1, CD95, TNFRSF 6
- Molecular Weight
- 45 kDa
-
You are here:
