Suppressor of Ty 4 Homolog 1 (SUPT4H1) (Middle Region) Peptide
SUPT4H1
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN984633
Quick Overview for Suppressor of Ty 4 Homolog 1 (SUPT4H1) (Middle Region) Peptide (ABIN984633)
Target
SUPT4H (SUPT4H1)
(Suppressor of Ty 4 Homolog 1 (SUPT4H1))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Sequence
- NCDAYLQMKG NREMVYDCTS SSFDGIIAMM SPEDSWVSKW QRVSNFKPGV
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-SUPT4H1 Antibody(ARP38258_P050),. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- SUPT4H (SUPT4H1) (Suppressor of Ty 4 Homolog 1 (SUPT4H1))
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Background
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SUPT4H1 is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Alias Symbols: SPT4H, SUPT4H, SPT4
Protein Interaction Partner: ANXA5,HTATSF1,SUPT5H,XRCC5,SUPT5H
Protein Size: 117 -
Molecular Weight
- 13 kDa
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Gene ID
- 6827
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NCBI Accession
- NM_003168, NP_003159
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UniProt
- P63272
Target
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