Browse our anti-AKT (AKT1) Antibodies

Human V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. This study presents further evidence to show that TRPM4 regulates beta-catenin signaling and enhances the proliferation of prostate cancer cell lines, through a calcium-dependent regulation of Akt1 and GSK-3beta activity.

2. miRNA-520a reduced hepatitis b virus transcription and replication by reducing AKT levels.

3. DDX5 promotes hepatocellular carcinoma tumorigenesis via Akt signaling pathway.

4. High AKT1 expression is associated with cholangiocarcinoma metastasis.

5. These results highlight an Arf GAP-independent function of ARAP2 in regulating Akt activity.

6. High AKT expression is associated with metastasis of hepatocellular carcinoma.

7. Data show that Y-box binding protein-1 (YBX1) promoted tumorigenesis and progression in spinal chordoma via the epidermal growth factor receptor (EGFR)/proto-oncogene proteins c-akt (AKT) pathway.

8. High AKT1 expression is associated with gastric cancer.

9. he intronic variant, AKT1 rs3803304, may act as a predictive biomarker in the risk of developing breast cancer in the high altitude Ecuadorian mestizo population.

10. tumor cells incubated with LGRFYAASG-pen showed disruption of filamentous actin, focal adhesions and caveolae-mediated membrane trafficking, resulting in impaired cell adhesion and migration in vitro. These effects were paralleled by a decrease in the phosphorylation of both focal adhesion kinase (Fak) and protein kinase B (Akt).

11. 50 diffuse-type gastric tumors were analyzed for AKT1 exon 2 and 14 mutations. The novel mutations, 15553T >A and 25376C >G might affect the exonic splicing enhancers and silencers. Significant decline was observed in the S-phase population in the tumor cells with 15553T >A and 15579G >C mutations suggesting the arrest of G1 phase.

12. These results indicate that BSNQ and OSNQ induce apoptosis in human hepatoma Hep3B cells via ROS-mediated p38/MAPK, Akt and STAT3 signaling pathways, suggesting that these 1,4-naphthoquinone derivatives may provide promising new anticancer agents to treat HCC.

13. The optimal melatonin concentration (3 mM) significantly decreased the intracellular reactive oxygen species levels, caspase-3 activity and the percentage of both dead and apoptotic-like sperm cells and increased the vitality, progressive motility and total motility and AKT phosphorylation compared with the control group.

14. The findings indicate that SPRY4 and SPRY4-IT1 may act as oncogenes in testicular germ cell tumors via activation of the PI3K/Akt signaling pathway.

15. Results suggest that transient receptor potential vanilloid 4 (TRPV4) accelerates glioma migration and invasion through the AKT/Rac1 signaling, and TRPV4 might be considered as a potential target for glioma therapy.

16. Data indicate a regulatory mechanism underlying drug resistance and suggest that tribbles homologue 2 (TRIB2) functions as a regulatory component of the PI3K network, activating AKT in cancer cells.

17. findings indicated that shikonin inhibits proliferation and promotes apoptosis in human endometrioid endometrial cancer (EEC) cells by modulating the miR-106b/PTEN/AKT/mTOR signaling pathway, suggesting shikonin could act a potential therapeutic agent in the EEC treatment.

18. SIRT6 inhibited proliferation, migration, and invasion of colon cancer cells by up-regulating PTEN expression and down-regulating AKT1 expression.

19. LHPP suppresses cell proliferation and metastasis in cervical cancer, and promotes apoptosis by suppressing AKT activation.

20. Data show that activated proto-oncogene protein Akt (AKT) directly phosphorylates Fas associated factor 1 (FAF1) reduces FAF1 at the plasma membrane and results in an increase in TGF-beta type II receptor (TbetaRII) at the cell surface.

Mouse (Murine) V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. CLOCK phosphorylation by AKT on Ser-845 regulates its nuclear translocation and the expression levels of certain core circadian genes in insulin-sensitive tissues.

2. Exosomes derived from pancreatic cancer cells induce insulin resistance in C2C12 myotube cells through the Pik3cd/Akt/FoxO1 pathway.

3. Endothelial Akt1 signaling is necessary for ischemic resolution post-injury and likely reflects the consequence of NO insufficiency critical for vascular repair.

4. Akt1 plays a critical role in regulating satellite cell proliferation during load-induced muscle hypertrophy.

5. PIP5K1alpha was found to be required for AKT activation and calcium release, both of which were important for skeletal muscle differentiation.

6. Kcnj13 mutants exhibit a shorter trachea as well as defective smooth muscle cell alignment and polarity. Kcnj13 is essential to maintain ion homeostasis in tracheal smooth cells, which is required for actin polymerization and is mediated, at least in part, through activation of the actin regulator, AKT.

7. M-CSF-evoked ERK1/2 activation was decreased, whereas AKT activation was enhanced in SHP2-deficient BMMs. ERK1/2, via its downstream target RSK2, mediates this negative feedback by negatively regulating phosphorylation of M-CSF receptor at Tyr721 and, consequently, its binding to p85 subunit of PI3K and PI3K activation.

8. Site-directed mutagenesis of Akt at Cys224 revealed that S-nitrosylation at this site was pivotal for the reduced phosphorylation at Akt Ser473, which led to impaired Akt signaling. Furthermore, on HHcy challenge, as compared with GSNOR(+/+)ApoE(-/-) littermate controls, GSNOR(-/-)ApoE(-/-) double knockout mice showed reduced T-cell activation with concurrent reduction of atherosclerosis.

9. the present study suggested that Pulsed electromagnetic fields (PEMFs) reduced osteoclast formation from RAW264.7 macrophages via inhibition of the Akt/mTOR signaling pathway. These findings provided novel insight into the mechanisms through which PEMFs suppress osteoclast differentiation.

10. findings uncover a new function of p53 in the regulation of Akt signaling and reveal how p53, ASS1, and Akt are interrelated to each other.

11. Here, we describe a role for PI3K/AKT in the regulation of TRF1, an essential component of the shelterin complex. PI3K and AKT chemical inhibitors reduce TRF1 telomeric foci and lead to increased telomeric DNA damage and fragility. TRF1 is phosphorylated by AKT regulating TRF1 protein stability and TRF1 binding to telomeric DNA in vitro and are important for in vivo TRF1 telomere location and cell viability.

12. High AKT1 expression is associated with cardiac hypertrophy.

13. CTRP1 protected against Dox-induced cardiotoxicity via activation of AKT

14. Noise exposure led to enhanced JNK phosphorylation and IRS1 serine phosphorylation as well as reduced Akt phosphorylation in skeletal muscles in response to exogenous insulin stimulation.

15. WT PDCD5 competitively inhibited interaction between histone deacetylase 3 (HDAC3) and AKT, but PDCD5(L6R), an HDAC3-binding-deficient mutant, did not. Knockdown of PDCD5 accelerated HDAC3-AKT interaction, AKT and eNOS phosphorylation, and nitric oxide (NO) production

16. both NAD and NADH significantly increased the intracellular ATP levels of BV2 microglia, which were attenuated by SIRT2 siRNA, the SIRT2 inhibitor AGK2, and the phosphatidylinositol 3-kinase/Akt inhibitor LY294002. Results suggested that SIRT2 mediates the NAD-induced and NADH-induced increase in Akt phosphorylation in BV2 microglia.

17. CYP2E1-induced oxidative stress may be responsible for ethanol-induced suppression of Akt phosphorylation and pharmacological modulation of Akt in liver may be an effective strategy for the treatment of ethanol-induced fatty liver.

18. The authors find in Mus musculus, each AKT isoform has a unique expression pattern in the hippocampus. AKT1, but not AKT2 or AKT3, is required for late long term potetiation (LTD) through regulating activity-induced protein synthesis. Interestingly, AKT activity inhibits mGluR-LTD, with overlapping functions for AKT1 and AKT3.

19. At 3days after the first tamoxifen injection, Akt1(-/-)/iAkt2 KO hearts showed decreased expression of connexin43 (Cx43) and connexin-interacting protein zonula occludens-1 (ZO-1). Furthermore, Akt1/2 silencing significantly decreased both Cx43 and ZO-1 expression

20. AKT1 plays an important role in the underlying pathomechanism.

Fruit Fly (Drosophila melanogaster) V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. The metabolic defects of cycG mutant animals are abrogated by a concomitant loss of Wdb, CycG presumably influences Akt1 activity at the PP2A nexus; Well rounded (Wrd), another B' subunit of PP2A in Drosophila, binds CycG similar to Wdb, and that its loss ameliorates some, but not all, of the metabolic defects of cycG mutants.

2. Our findings demonstrated that lovastatin restored LRRK2-G2019S neurite degeneration by augmenting Akt/NRF2 pathway and inhibiting downstream GSK3b activity, which decreased phospho-tau levels. We suggested that lovastatin is a potential disease-modifying agent for LRRK2-G2019S parkinsonism.

3. subtle manipulation of foxo through Akt1 can enhance survival during adverse nutrient conditions in Drosophila.

4. The developmental delay of these novel Akt1 hypomorphs results in a latent phenotype uncovered by generation of somatic clones

5. these data show that Drosophila Trbl has a conserved role to bind Akt and block Akt-mediated insulin signaling, and implicate Trib proteins as novel sites of signaling pathway integration that link nutrient availability with cell growth and proliferation

6. AKT1 and caspase-dependent regulation of Acn stability adjusts basal autophagy levels.

7. Akt1 governs two critical elements of synapse development, neurotransmitter receptor localization, and postsynaptic membrane elaboration

8. Tsc2 mutants showed a dramatic decrease in the levels of phosphorylated Akt, and interestingly, Akt mutants phenocopied Tsc2 mutants, leading to the hypothesis that Tsc2 and Akt might work via the same genetic pathway to regulate synapse growth.

9. Hippo signaling not only blocks cell division and promotes apoptosis, but also regulates cellular growth by inhibiting the Akt pathway activity.

10. Regeneration of Drosophila sensory neuron axons and dendrites is regulated by the Akt pathway involving Pten and microRNA bantam.

11. Overexpression of Akt enhanced Sindbis virus replication.

12. Perturbation of the Akt/Gsk3-beta signalling pathway is common to Drosophila expressing expanded untranslated CAG, CUG and AUUCU repeat RNAs.

13. dAkt activation under wild-type conditions is defined by feedback inhibition that depends on TOR Complex 1

14. Studies indicate that knockdown of dGirdin in the Drosophila wing imaginal disc cells resulted in reduction of cell size and this was enhanced by half reduction of the Akt gene dose.

15. dS6K activity is dependent on the Drosophila homologue of the phosphoinositide-dependent protein kinase 1, dPDK1, demonstrating that both dPDK1, as well as dTOR, mediated dS6K activation is phosphatidylinositide-3,4,5-trisphosphate (PIP3)-independent.

16. A mutation in the pleckstrin homology (PH) domain of Akt that reduces its affinity for PIP3 sufficed to rescue the lethality of flies devoid of PTEN activity.

17. stimulates growth by phosphorylating the tuberous sclerosis complex 2 (Tsc2) tumour suppressor and inhibiting formation of a Tsc1-Tsc2 complex

18. cell growth control by PTEN and it's ability to regulate known cell size regulators involved in protein translation (review)

19. In Drosophila cells, Drosophila protein kinase B is required for the activation of Drosophila p70 ribosomal S6 protein kinase by insulin.

20. InR regulates cell growth and proliferation through the dAkt pathway.

Cow (Bovine) V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. The role of the PI3K/Akt/mTOR pathway in inflammatory regulation is independent of the activation of TLRs/NF-kappaB. Cross-talk between PI3K/Akt/mTOR and TLRs/NF-kappaB signaling pathways promote inflammation.

2. Thus, activation of STAT3 and inactivation of AKT signaling are involved in structural regression of the corpus luteum.

3. the measurement of levels of PI3K-Akt pathway components in FCs from ovarian follicles carrying oocytes with distinct developmental competences is a useful tool to identify putative molecular pathways involved in the acquisition of oocyte competence.

4. These results demonstrate that activation of AKT is required for gonadotropin regulation of CTNNB1 accumulation and subsequent ovarian E2 production.

5. Caveolin-1 scaffolding domain residue phenylalanine 92 modulates Akt signaling

6. TG2 contributes to 5-hydroxytryptamine-induced distal pulmonary artery smooth muscle cell proliferation via promotion of AKT signaling, likely via its serotonylation.

7. results suggest that PI3K-Akt activity is important for the internalization of S. aureus and phosphorylation of GSK-3alpha, GSK-3beta, and NF-kappaB.

8. The current study was designed to determine mechanisms underlying 20-hydroxyeicosatetraenoic acid -stimulated nitric oxide (NO) release, and particularly the role of NADPH oxidase, reactive oxygen species, and PI3-kinase in stimulated NO release.

9. PI3K/Akt and p53 are redox-regulated in bovine aortic endothelial cells exposed to hydrogen peroxide

10. Thus our data demonstrate that hypoxia-induced adventitial fibroblast proliferation requires activation and interaction of PI3K, Akt, mTOR, p70S6K, and ERK1/2.

11. Gab1 tyrosine phosphorylation is stimulated by flow shear stress to mediate protein kinase B and endothelial nitric-oxide synthase activation in endothelial cells

12. Losartan metabolite stimulates eNOS phosphorylation and suppresses tumor necrosis factor alpha-induced endothelial cell apoptosis by activating AKT1.

13. prostaglandin F2alpha phosphorylates TSC2 and activates mTOR and ribosomal protein S6 kinase signaling in an AKT-independent manner

14. Results suggest that the mitogenic response to FGF-2 in vivo in the corneal endothelial layer may be inhibited by TGF-beta2-induced suppression of the PI3-kinase/AKT signaling pathway.

15. These results suggest that bGPR40 mediates LCFA signaling in mammary epithelial cells and thereby plays an important role in cell proliferation and survival.

16. Data show that laminar shear stress stimulates vascular smooth muscle cell apoptosis via the Akt pathway.

17. The intracellular mechanism of action of CART in regulation of FSH-induced MAPK signaling.

18. AMPK and AKT are both downstream of PI3K and that AKT activation is partially dependent on AMPK. The interrelationship between AMPK and AKT, although known to be individually important in mediating VEGF activation of eNOS, is clearly characterized.

Xenopus laevis V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. These findings highlight novel and essential roles of PFKFB4 activity in later stages of neural crest (NC) development that are wired into the NC gene regulatory network.

Pig (Porcine) V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. Studied the effects of microRNA-27a on myogenin expression and the Akt/FoxO1 signal pathway during porcine myoblast differentiation. Overexpression of miR-27a suppressed myogenin expression during porcine myoblast differentiation, whereas inhibition of miR-27a promoted the mRNA and protein expression levels of myogenin; overexpression of miR-27a decreased the level of P-Akt/Akt and increased the protein level of FoxO1.

2. SCF is a critical regulatory factor for conceptus development and implantation during pregnancy in pigs.

3. These results indicate glycine enhances muscle protein mass under an inflammatory condition. The beneficial roles of glycine on the muscle are closely associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing the activation of TLR4 and/or NOD2 signaling pathways.

4. Data show that homocysteine (Hcy) can ameliorate the endothelium-independent hypoxic coronary vasoconstriction, in which the inhibition of PI3K/Akt signaling pathway may be involved.

5. In pigs, lactose synthesis was significantly elevated with the increase of milk production and AKT1 could positively regulate lactose synthesis.

6. In conclusion, our observations reveal that PRRSV triggers the activation of FAK-PI3K-AKT-Rac1 signaling pathway to facilitate its entry into cells.

7. Host PI3K and Akt1 play a role in viral gene expression, leading to an increase in porcine reproductive and respiratory syndrome virus replication.

8. Activity of AKT is not essential for induction of germinal vesicle breakdown in porcine oocytes but plays a substantial role during progression of meiosis to MI/MII-stage.

9. IL-4 induced activation of Akt/SREBP-1/lipid biosynthesis in EC, resulting in protection against membrane attack complex and melittin, in association with mitochondrial protection.

10. findings show that megalin is the sensor that determines whether cells will be protected or injured by albumin; it binds protein kinase B (PKB) in a D-3-phosphorylated phospholipid-insensitive manner, anchoring PKB in the luminal plasma membrane [

11. protein kinase B (PKB/Akt)was localized in the granulosa cells of primordial follicles and in the basal layers of the granulosa cells of preantral and antral follicles, but were not localized in atretic follicles and corpora lutea

12. Akt signaling in porcine patellofemoral joint cartilage is dependent upon frequency of loading, cartilage zone, and the time interval between loading and cartilage harvest.

13. in VSMCs exposed to hyperglycemia, IGF-I stimulation of Shc facilitates the transfer of Grb2 to p85 resulting in enhanced PI3K activation and AKT phosphorylation leading to enhanced cell proliferation and migration

14. There was no correlation of infarct size with expression or phosphorylation of AKT in ischemic postconditioning.

Arabidopsis thaliana V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K(+) uptake/leakage to modulate AKT1-mediated low potassium responses in Arabidopsis.

2. results suggest that NO decreases K(+) absorption by promoting the synthesis of vitamin B6 PLP, which further represses the activity of K(+) channel AKT1 in Arabidopsis.

3. Examination of the athak5 atakt1 double mutant, revealing novel aspects of an uptake system as yet unidentified by genetic means.

4. AKT1 is regulated by CIPK23 in guard cells and is involved in water stress responses.

5. These findings provide further insights into the signaling network consisting of CBL-CIPK-PP2C interactions in the activation of the AKT1 channel.

6. Electrophysiological results showed that AtKC1 inhibited the AKT1-mediated inward K(+) currents and negatively shifted the voltage dependence of AKT1 channels.

7. AtHAK5 and AKT1 are vital for plant growth and development at low K+ concentrations.

8. In the range between 0.01 and 0.05 mM K+ AtHAK5 and AtAKT1 are the only contributors to K+ acquisition. At higher K+ concentrations, unknown systems come into operation and participate together with AtAKT1 in low-affinity K+ uptake.

9. Potassium transport and gene expression in A. thaliana deficient at this site.

10. CIPK23 directly phosphorylates the K+ transporter AKT1

11. Data show that interacting calcium sensors (CBL1 and CBL9) together with CIPK23, but not either alone, activated the AKT1 channel in a Ca(2+)-dependent manner, connecting the Ca(2+) signal to K(+) uptake through activation of a K(+) channel.

12. AtAKT1 mediates K+ absorption at high concentrations, and remains active in the presence of NH4+ although activity is reduced.

13. AtKC1-AKT1 channels in Arabidopsis roots facilitate growth under K+-limiting conditions

Caenorhabditis elegans (C. elegans) V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. the LIN-28/let-7/AKT/DAF-16 axis is a program that plays an important role in balancing reproduction and somatic maintenance.

2. this study shows that akt-1 and akt-2 negatively regulate DNA-damage-induced apoptosis in the C. elegans germline and the antiapoptotic activity of akt-1 is independent of its target gene daf-16 but dependent on cep-1/p53.

3. Modulation of pptr-1 affects insulin/IGF-1 signaling pathway-associated phenotypes including life span, dauer, stress resistance, and fat storage; study shows that PPTR-1 functions by regulating worm AKT-1 phosphorylation at Thr 350.

Goat V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. Exogenous AKT was transcribed, and AKT was overexpressed, inducing the phosphorylation of p70S6K (Thr389) and 4E-BP1 (Thr37/46) in goat fetal fibroblasts.

Guinea Pig V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. findings suggested that the expressions of the cardiac CACNA1C were under the CLOCK-BMAL1 regulation, probably through the PI3K-Akt signal pathway

Zebrafish V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) interaction partners

1. This study has identified Akt as a novel intracellular pathway required for neural crest differentiation.

2. Overexpression of human Akt1 enhances adipogenesis and leads to lipoma formation in zebrafish.