ATP-Dependent DNA Helicase Rep (AA 1-673), (full length) protein (GST tag)

Catalog No. ABIN1095485

Product Details

Target: ATP-Dependent DNA Helicase Rep

Protein Type: Recombinant

Protein Characteristics: AA 1-673, full length

Origin: E. coli

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: GST tag

Application: SDS-PAGE (SDS), ELISA

Sequence: MRLNPGQQQA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RGCGYQARHI AAVTFTNKAA REMKERVGQT LGRKEARGLM ISTFHTLGLD IIKREYAALG MKANFSLFDD TDQLALLKEL TEGLIEDDKV LLQQLISTIS NWKNDLKTPS QAAASAIGER DRIFAHCYGL YDAHLKACNV LDFDDLILLP TLLLQRNEEV RKRWQNKIRY LLVDEYQDTN TSQYELVKLL VGSRARFTVV GDDDQSIYSW RGARPQNLVL LSQDFPALKV IKLEQNYRSS GRILKAANIL IANNPHVFEK RLFSELGYGA ELKVLSANNE EHEAERVTGE LIAHHFVNKT QYKDYAILYR GNHQSRVFEK FLMQNRIPYK ISGGTSFFSR PEIKDLLAYL RVLTNPDDDS AFLRIVNTPK REIGPATLKK LGEWAMTRNK SMFTASFDMG LSQTLSGRGY EALTRFTHWL AEIQRLAERE PIAAVRDLIH GMDYESWLYE TSPSPKAAEM RMKNVNQLFS WMTEMLEGSE LDEPMTLTQV VTRFTLRDMM ERGESEEELD QVQLMTLHAS KGLEFPYVYM VGMEEGFLPH QSSIDEDNID EERRLAYVGI TRAQKELTFT LCKERRQYGE LVRPEPSRFL LELPQDDLIW EQERKVVSAE ERMQKGQSHL ANLKAMMAAK RGK

Characteristics: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication, it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction.

Purity: 90 %

Application Details

Comment:

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS buffer, 20mM GSH

Storage: -20 °C

References

Gilchrist, Denhardt: "Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD." in: Nucleic acids research, Vol. 15, Issue 2, pp. 465-75, (1987) (PubMed).

Target Details

Target: ATP-Dependent DNA Helicase Rep

Molecular Weight: 104.4 kD

UniProt: P09980