EIF3F Protein (AA 2-250, partial) (GST tag)

Catalog No. ABIN1046992

Product Details

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Protein Type: Recombinant

Protein Characteristics: AA 2-250, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EIF3F protein is labelled with GST tag.

Application: ELISA

Sequence: ATPAVPVSAP PATPTPVPAA APASVPAPTP APAAAPVPAA APASSSDPAA AAAATAAPGQ TPASAQAPAQ TPAPALPGPA LPGPFPGGRV VRLHPVILAS IVDSYERRNE GAARVIGTLL GTVDKHSVEV TNCFSVPHNE SEDEVAVDME FAKNMYELHK KVSPNELILG WYATGHDITE HSVLIHEYYS REAPNPIHLT VDTSLQNGRM SIKAYVSTLM GVPGRTMGVM FTPLTVKYAY YDTERIGVD

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, (2003) (PubMed).

Asano, Vornlocher, Richter-Cook, Merrick, Hinnebusch, Hershey: "Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly." in: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 27042-52, (1997) (PubMed).

Target Details

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Alternative Name: Eukaryotic translation initiation factor 3 subunit F protein (EIF3F Products)

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 53.2 kD

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization