Interferon gamma Protein (IFNG) (AA 24-161, partial) (His tag)

Catalog No. ABIN1047217

Product Details

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Protein Characteristics: AA 24-161, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Interferon gamma protein is labelled with His tag.

Application: ELISA

Sequence: QDPYVKEAEN LKKYFNAGHS DVADNGTLFL GILKNWKEES DRKIMQSQIV SFYFKLFKNF KDDQSIQKSV ETIKEDMNVK FFNSNKKKRD DFEKLTNYSV TDLNVQRKAI HELIQVMAEL SPAAKTGKRK RSQMLFRG

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Nishi, Fujita, Nishi-Takaoka, Saito, Matsumoto, Sato, Oka, Itoh, Yip, Vilcek: "Cloning and expression of a novel variant of human interferon-gamma cDNA." in: Journal of biochemistry, Vol. 97, Issue 1, pp. 153-9, (1985) (PubMed).

Gray, Leung, Pennica, Yelverton, Najarian, Simonsen, Derynck, Sherwood, Wallace, Berger, Levinson, Goeddel: "Expression of human immune interferon cDNA in E. coli and monkey cells." in: Nature, Vol. 295, Issue 5849, pp. 503-8, (1982) (PubMed).

Gray, Goeddel: "Structure of the human immune interferon gene." in: Nature, Vol. 298, Issue 5877, pp. 859-63, (1982) (PubMed).

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: Interferon gamma protein (IFNG Products)

Synonyms: IFG Protein, IFI Protein, IFN-g Protein, Ifg Protein, IFNG2 Protein, IFN-gamma Protein, IFN-G Protein, IFNG Protein, IFNgamma Protein, TCRalpha Protein, INF-G Protein, ifng Protein, interferon gamma Protein, interferon, gamma 1-2 Protein, IFNG Protein, Ifng Protein, ifng1-2 Protein

Background: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Molecular Weight: 20.4 kD

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy