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IGF-r3 (AA 49-118) (Active) Protein

Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Func Active
Catalog No. ABIN1097098
  • Target
    IGF-r3
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 49-118
    Origin
    Human
    Source
    Escherichia coli (E. coli)
    Application
    Functional Studies (Func)
    Purpose
    Recombinant Human LR3 Insulin-Like Growth Factor I/LR3-IGF-1 (MG)
    Sequence
    MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
    Characteristics
    Recombinant Human LR3-IGF-1 (MG) is produced with our E. coli expression system. The target protein is expressed with sequence (G49-A118) of Human IGF1 R3.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Less than 0.05 ng/μg (0.5 IEU/μg) as determined by LAL test
  • Comment

    Biological activity: ED50 is greater than 200 ng/ml as determined by an IGF binding protein assay.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is not recommended to reconstitute to a concentration less than 100 μg/mL.
    Dissolve the lyophilized protein in 500 mM Acetic Acid.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Buffer
    Lyophilized from a 0.2 μm filtered solution of 300 mM HAc-NaAc, pH 6.5.
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    Storage
    4 °C/-20 °C/-80 °C
    Storage Comment
    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
    Expiry Date
    3 months
  • Target
    IGF-r3
    Background
    Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro.
    Alternative Names: Insulin-Like Growth Factor I, IGF-I, Mechano Growth Factor, MGF, Somatomedin-C, IGF1, IBP1
    Molecular Weight
    9.1 kDa
    UniProt
    P05019
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