PEG-G-CSF protein (His tag)
Origin: Mouse
Host: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- PEG-G-CSF
- Protein Type
- Recombinant
- Origin
- Mouse
-
Source
- Human Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Mouse Noggin/NOG (C-6His)
- Sequence
- QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGPAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSCHHHHH H
- Characteristics
- Recombinant Human G-CSF produced in E. coli is a single. non-glycosylated. polypeptide chain containing 175 amino acids and having a molecular mass of 18.800 Dalton. PEG-GCSF is manufactured by attaching a 20.000 Dalton methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of G-CSF having a total molecular mass of 38.800 Dalton.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Comment
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Gene Name: CSF3 C17orf33 GCSF
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PEG-G-CSF
- Molecular Weight
- 23.9 kDa
- UniProt
- P97466
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