PykF protein (His tag)
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
1 image
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- Target
- PykF
- Protein Type
- Recombinant
- Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
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- Comment
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Synonyms: pyruvate kinase I, ECK1672, JW1666
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- PykF
- Background
- PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E.coli pykF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 53.3kDa (494aa)
- NCBI Accession
- NP_416191
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