PykF protein (His tag)

Catalog No. ABIN1098253

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for PykF protein (His tag) (ABIN1098253)

Target: PykF

Protein Type: Recombinant

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Purification tag / Conjugate: His tag

Purification: purified by chromatography

Application Details

Comment:

Synonyms: pyruvate kinase I, ECK1672, JW1666

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: PykF

Background: PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E.coli pykF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 53.3kDa (494aa)

NCBI Accession: NP_416191