POLR2J3 Protein (His tag)

Catalog No. ABIN1098407

Product Details

Target: POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This POLR2J3 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Purity: > 95 % by SDS - PAGE

Application Details

Comment:

Synonyms: DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol,1 MM DTT

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))

Alternative Name: POLR2J3 (POLR2J3 Products)

Background: DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 15.5 kDa (138aa) confirmed by MALDI-TOF

NCBI Accession: NP_001091084