KERA Protein (AA 253-351) (GST tag)

Catalog No. ABIN1308536

Product Details

Target: KERA (Keratocan (KERA))

Protein Type: Recombinant

Protein Characteristics: AA 253-351

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This KERA protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: KERA (Human) Recombinant Protein (Q01)

Sequence: GLPSRGFDVS SILDLQLSHN QLTKVPRISA HLQHLHLDHN KIKSVNVSVI CPSPSMLPAE RDSFSYGPHL RYLRLDGNEI KPPIPMALMT CFRLLQAVI

Characteristics: Human KERA partial ORF ( NP_008966, 253 a.a. - 351 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Zhang, Mao, Schwend, Littlechild, Conrad: "Resistance of corneal RFUVA–cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases." in: Investigative ophthalmology & visual science, Vol. 54, Issue 2, pp. 1014-25, (2013) (PubMed).

Zhang, Conrad, Conrad: "Effects of ultraviolet-A and riboflavin on the interaction of collagen and proteoglycans during corneal cross-linking." in: The Journal of biological chemistry, Vol. 286, Issue 15, pp. 13011-22, (2011) (PubMed).

Target Details

Target: KERA (Keratocan (KERA))

Alternative Name: KERA (KERA Products)

Background: Full Gene Name: keratocan
Synonyms: CNA2,SLRR2B

Gene ID: 11081

NCBI Accession: NM_007035

Pathways: Glycosaminoglycan Metabolic Process