SMAD1 Protein (AA 1-465) (GST tag)
(2 references)-
- Target See all SMAD1 Proteins
- SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-465
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This SMAD1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- SMAD1 (Human) Recombinant Protein (P01)
- Sequence
- MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE LEKALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH HELKPLECCE FPFGSKQKEV CINPYHYKRV ESPVLPPVLV PRHSEYNPQH SLLAQFRNLG QNEPHMPLNA TFPDSFQQPN SHPFPHSPNS SYPNSPGSSS STYPHSPTSS DPGSPFQMPA DTPPPAYLPP EDPMTQDGSQ PMDTNMMAPP LPSEINRGDV QAVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT DPSNNKNRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD SSIFVQSRNC NYHHGFHPTT VCKIPSGCSL KIFNNQEFAQ LLAQSVNHGF ETVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV LTQMGSPHNP ISSVS
- Characteristics
- Human SMAD1 full-length ORF ( AAH01878.1, 1 a.a. - 465 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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SMAD, Mothers Against DPP Homolog 1 (SMAD1) (AA 1-465) protein (His tag)
custom-made SMAD1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SMAD, Mothers Against DPP Homolog 1 (SMAD1) (AA 1-250) protein (His tag)SMAD1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD, Mothers Against DPP Homolog 1 (SMAD1) (AA 1-110) protein (GST tag)SMAD1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 1 (SMAD1) (AA 1-465) protein (His tag)custom-made SMAD1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Simultaneous imaging and restoration of cell function using cell permeable peptide probe." in: Biomaterials, Vol. 35, Issue 24, pp. 6287-98, (2014) (PubMed).
: "Identification of substrates of SMURF1 ubiquitin ligase activity utilizing protein microarrays." in: Assay and drug development technologies, Vol. 8, Issue 4, pp. 471-87, (2010) (PubMed).
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: "Simultaneous imaging and restoration of cell function using cell permeable peptide probe." in: Biomaterials, Vol. 35, Issue 24, pp. 6287-98, (2014) (PubMed).
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- Target
- SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
- Alternative Name
- SMAD1 (SMAD1 Products)
- Background
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Full Gene Name: SMAD family member 1
Synonyms: BSP1,JV4-1,JV41,MADH1,MADR1 - Gene ID
- 4086
- Pathways
- Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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