WNT3A Protein (AA 1-385) (GST tag)
(1 reference)-
- Target See all WNT3A Proteins
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-385
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This WNT3A protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- WNT3A (Human) Recombinant Protein (P01)
- Sequence
- MAPLGYFLLL CSLKQALGSY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ FRGRRWNCTT VHDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWCCYVSCQ ECTRVYDVHT CKNPGSRAGN SAHQPPHPQP PVRFHPPLRR AGKVP
- Characteristics
- Human WNT3A full-length ORF ( AAI03922.1, 1 a.a. - 385 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) protein
WNT3A Origin: Human Host: Human Cells Recombinant >90 % as determined by reducing SDS-PAGE.
Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-352) protein (His-IF2DI Tag)WNT3A Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Blockage of a miR-21/EGFR regulatory feedback loop augments anti-EGFR therapy in glioblastomas." in: Cancer letters, Vol. 342, Issue 1, pp. 139-49, (2013) (PubMed).
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: "Blockage of a miR-21/EGFR regulatory feedback loop augments anti-EGFR therapy in glioblastomas." in: Cancer letters, Vol. 342, Issue 1, pp. 139-49, (2013) (PubMed).
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- Target
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- Alternative Name
- WNT3A (WNT3A Products)
- Background
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Full Gene Name: wingless-type MMTV integration site family, member 3A
Synonyms: MGC119418,MGC119419,MGC119420 - Gene ID
- 89780
- Pathways
- WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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