Periostin Protein (POSTN) (AA 24-783) (DYKDDDDK Tag)

Catalog No. ABIN1344268

Product Details

Target: Periostin (POSTN)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 24-783

Origin: Mouse

Source: CHO Cells

Purification tag / Conjugate: This Periostin protein is labelled with DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Purpose: Periostin (mouse) (rec.)

Cross-Reactivity: Mouse

Characteristics: Mouse periostin (aa 24-783) (isoform 5) is fused at the C-terminus to a FLAG®-tag.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Biological Activity Comment: Rescues sphere formation from periostin KO primary tumor cells at 50ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Periostin (POSTN)

Alternative Name: Periostin (POSTN Products)

Background:

Osteoblast-specific Factor 2, OSF-2, Postn

Periostin is a 90- kDa matricellular protein that consists of a typical signal sequence, followed by a cysteine-rich region, an EMI domain (protein-protein interactions), four tandem fasciclin-like domains that are responsible for integrin binding, and a C-terminal region. Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and in osteoblast recruitment, attachment and spreading. Periostin is also involved in many fundamental biological processes such as cell proliferation, cell invasion and angiogenesis. Periostin expression is increased by both transforming growth factor beta1 (TGF-beta1) and bone morphogenetic protein 2 (BMP-2). Changes in periostin expression are commonly detected in various cancers and pre-cancerous conditions, and periostin may be involved in regulating cancer cell activities that contribute to tumorigenesis, cancer progression and metastasis. Periostin has shown to be involved in many aspects of allergic inflammation, such as eosinophil recruitment, airway remodeling, development of a Th2 phenotype and increased expression of inflammatory mediators. It is evaluated as a biomarker for bronchial asthma and airway inflammation.

Molecular Weight: ~85kDa

UniProt: Q62009