ATG7 Protein (AA 1-703) (GST tag)

Catalog No. ABIN1345982

Product Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Protein Type: Recombinant

Protein Characteristics: AA 1-703

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This ATG7 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: ATG7 (Human) Recombinant Protein (P01)

Sequence: MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS SKVLDQYERE GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD ETI

Characteristics: Human ATG7 full-length ORF ( NP_006386.1, 1 a.a. - 703 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Han, Hou, Goldstein, Stolz, Watkins, Rabinowich: "A Complex between Atg7 and Caspase-9: A NOVEL MECHANISM OF CROSS-REGULATION BETWEEN AUTOPHAGY AND APOPTOSIS." in: The Journal of biological chemistry, Vol. 289, Issue 10, pp. 6485-97, (2014) (PubMed).

Target Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7 (ATG7 Products)

Background: Full Gene Name: ATG7 autophagy related 7 homolog (S. cerevisiae)
Synonyms: APG7-LIKE,APG7L,DKFZp434N0735,GSA7

Gene ID: 10533

NCBI Accession: NM_006395

Pathways: Response to Water Deprivation, Autophagy