GAD65 Protein (AA 1-585) (GST tag)
(2 references)-
- Target See all GAD65 (GAD2) Proteins
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-585
-
Origin
- Human
-
Source
- Wheat germ
- Purification tag / Conjugate
- This GAD65 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- GAD2 (Human) Recombinant Protein (P02)
- Sequence
- MASPGSGFWS FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG DAEKPAESGG SQPPRAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MMIARFKMFP EVKEKGMAAL PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQNCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH VDKCLELAEY LYNIIKNREG YEMVFDGKPQ HTNVCFWYIP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
- Characteristics
- Human GAD2 full-length ORF ( NP_000809.1, 1 a.a. - 585 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
- Top Product
- Discover our top product GAD2 Protein
-
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (His tag)
custom-made GAD2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (His tag)custom-made GAD2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) proteinGAD2 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein (GST tag,His tag)GAD2 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein (GST tag)GAD2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
-
: "Essential roles of insulin expression in Aire+ tolerogenic dendritic cells in maintaining peripheral self-tolerance of islet ?-cells." in: Cellular immunology, Vol. 273, Issue 2, pp. 115-23, (2012) (PubMed).
: "Thymus-specific deletion of insulin induces autoimmune diabetes." in: The EMBO journal, Vol. 28, Issue 18, pp. 2812-24, (2009) (PubMed).
-
: "Essential roles of insulin expression in Aire+ tolerogenic dendritic cells in maintaining peripheral self-tolerance of islet ?-cells." in: Cellular immunology, Vol. 273, Issue 2, pp. 115-23, (2012) (PubMed).
-
- Target
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Alternative Name
- GAD2 (GAD2 Products)
- Background
-
Full Gene Name: glutamate decarboxylase 2 (pancreatic islets and brain, 65 kDa)
Synonyms: GAD65,MGC161605,MGC161607 - Gene ID
- 2572
- NCBI Accession
- NM_000818
-
