PHGDH Protein (AA 2-251, partial) (GST tag)

Catalog No. ABIN7479282

Product Details

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Protein Type: Recombinant

Protein Characteristics: AA 2-251, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PHGDH protein is labelled with GST tag.

Application: ELISA

Sequence: AFANLRKVLI SDSLDPCCRK ILQDGGLQVV EKQNLSKEEL IAELQDCEGL IVRSATKVTA DVINAAEKLQ VVGRAGTGVD NVDLEAATRK GILVMNTPNG NSLSAAELTC GMIMCLARQI PQATASMKDG KWERKKFMGT ELNGKTLGIL GLGRIGREVA TRMQSFGMKT IGYDPIISPE VSASFGVQQL PLEEIWPLCD FITVHTPLLP STTGLLNDNT FAQCKKGVRV VNCARGGIVD EGALLRALQS

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 95 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Target Details

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: D-3-phosphoglycerate dehydrogenase protein (PHGDH Products)

Background: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.

Molecular Weight: 54.2 kD

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect