Topoisomerase I Protein (AA 2-767) (His tag)
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- Target See all Topoisomerase I (TOP1) Proteins
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-767
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Origin
- Rat
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Source
- Yeast
- Purification tag / Conjugate
- This Topoisomerase I protein is labelled with His tag.
- Application
- ELISA
- Sequence
- SGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE KKHKEKEKTK HKDGSSDKHK DKHKDRDKEK RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD EPEDDGYFAP PKEDIKPLKR PRDEDDADYK PKKIKTEDIK KEKKRKLEEE EDGKLKKPKN KDKDKKVAEP DNKKKKAKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPEGV KFYYDGKVMK LSPKAEEVAT FFAKMLDHEY TTKEIFRKNF FKDWRKEMTN DEKNTITNLS KCDFTQMSQY FKAQSEARKQ MSKEEKLKIK EENEKLLKEY GFCVMDNHRE RIANFKIEPP GLFRGRGNHP KMGMLKRRIM PEDIIINCSK DAKVPSPPPG HKWKEVRHDN KVTWLVSWTE NIQGSIKYIM LNPSSRIKGE KDWQKYETAR RLKKCVDKIR NQYREDWKSK EMKVRQRAVA LYFIDKLALR AGNEKEEGET ADTVGCCSLR VEHINLHPEL DGQEYVVEFD FPGKDSIRYY NKVPVEKRVF KNLQLFMENK QPEDDLFDRL NTGILNKHLQ DLMEGLTAKV FRTYNASITL QQQLKELTAP DENVPAKILS YNRANRAVAI LCNHQRAPPK TFEKSMMNLQ SKIDAKKDQL ADARKDLKSA KADAKVMKDA KTKKVVESKK KAVQRLEEQL MKLEVQATDR EENKQIALGT SKLNYLDPRI TVAWCKKWGV PIEKIYNKTQ REKFAWAIDM TDEDYEF
- Specificity
- Rattus norvegicus (Rat)
- Characteristics
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
- Purity
- > 90 %
- Top Product
- Discover our top product TOP1 Protein
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- Comment
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The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 0.2-2 mg/mL
- Buffer
- Tris-based buffer, 50 % glycerol
- Handling Advice
- Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.
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- Target
- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
- Alternative Name
- DNA topoisomerase 1 (Top1) (TOP1 Products)
- Target Type
- Viral Protein
- Background
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Recommended name: DNA topoisomerase 1.
EC= 5.99.1.2.
Alternative name(s): DNA topoisomerase I - UniProt
- Q9WUL0
- Pathways
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
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