CA2 Protein (AA 1-260) (His tag)

Catalog No. ABIN2180674

Product Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Protein Type: Recombinant

Protein Characteristics: AA 1-260

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CA2 protein is labelled with His tag.

Sequence: AA 1-260

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30 kDa. The protein migrates as 30 kDa under reducing (R) condition (SDS-PAGE).

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Wang, Zhou: "Dual-Valve and Counter-Flow Surface Plasmon Resonance." in: Analytical chemistry, Vol. 90, Issue 8, pp. 4972-4977, (2019) (PubMed).

Target Details

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase II (CA2 Products)

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans. Carbonic anhydrase II (CA2) is also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Molecular Weight: 30.1 kDa

NCBI Accession: NP_000058