HSA Protein (AA 25-609) (His tag)

Catalog No. ABIN2181228

Product Details

Target: HSA (Human Serum Albumin (HSA))

Protein Type: Recombinant

Protein Characteristics: AA 25-609

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HSA protein is labelled with His tag.

Sequence: AA 25-609

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 67.3 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Siaw, Raghunath, Dyer: "Peripheral Protein Unfolding Drives Membrane Bending." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 34, Issue 28, pp. 8400-8407, (2019) (PubMed).

Happonen, Hauri, Svensson Birkedal, Karlsson, de Neergaard, Khakzad, Nordenfelt, Wikström, Wisniewska, Björck, Malmström, Malmström: "A quantitative Streptococcus pyogenes-human protein-protein interaction map reveals localization of opsonizing antibodies." in: Nature communications, Vol. 10, Issue 1, pp. 2727, (2019) (PubMed).

Target Details

Target: HSA (Human Serum Albumin (HSA))

Alternative Name: HSA (HSA Products)

Background: Serum albumin (SA) is also known as ALB, which is the main protein of plasma and has a good binding capacity for water,Ca2+,Na+,K+,fatty acids,hormones, bilirubin and drugs.The main function of SA is the regulation of the colloidal osmotic pressure of blood. As Major zinc transporter in plasma, SA typically binds about 80 % of all plasma zinc. A variant structure of albumin could lead to increased binding of zinc resulting in an asymptomatic augmentation of zinc concentration in the blood. Defects in serum albumin can cause familial dysalbuminemic hyperthyroxinemia which is a form of euthyroid hyperthyroxinemia that is due to increased affinity of serum albumin for T4. It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

Molecular Weight: 67.4 kDa

NCBI Accession: NP_000468