T4 SSB (Active) Protein

Catalog No. ABIN2452207

Recombinant protein expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for T4 SSB (Active) Protein (ABIN2452207)

Target: T4 SSB

Protein Type: Recombinant

Biological Activity: Active

Origin: Bacteriophage T4

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % of protein determined by SDS-PAGE (CBB staining)

Product Details

Characteristics: The absence of endonucleases and exonucleases was confirmed.

Application Details

Application Notes: 1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
2. Increase specificity and yields of long PCR products

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Bittner, Burke, Alberts: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).

Alberts, Frey: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." in: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).

Target Details

Target: T4 SSB

Target Type: Phage Protein

Background: T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.

UniProt: P03695