TM Pst1 Protein

Catalog No. ABIN2469152

Product Details

Target: TM Pst1

Protein Type: Recombinant

Origin: Saccharomyces cerevisiae

Source: Yeast (Saccharomyces Cerevisiae)

Application: Screening Assay (ScA), ELISA, Western Blotting (WB)

Purity: ~95 %

Application Details

Application Notes: This recombinant protein can be used for WB, ELISA, and other assays to screen and identify mannose-specific antibodies.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Lot specific, TBS or PBS

Handling Advice: Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -80 °C

Storage Comment: Store in working aliquots at -70 °C.

Target Details

Target: TM Pst1

Background: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1). Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2). Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at around 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (?och1 ?mnn1 ?mnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified glycan-specific PGT antibodies (3). Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows high affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4).

Molecular Weight: 100 kDa on SDS-PAGE

Gene ID: 851625

OMIM: 17380259

UniProt: Q12355