Nuclear Factor kappa B p50 (NFkB p50) (full length ) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
GS, ELISA
1 image
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- Target
- Nuclear Factor kappa B p50 (NFkB p50)
- Protein Type
- Recombinant
- Protein Characteristics
- full length
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Gel Shift (GS), ELISA
- Characteristics
- Recombinant NFκB p50 protein was expressed from a full-length cDNA clone in E. coli (accession number (P19838). Protein was purified by affinity chromatography, followed by gel filtration.
- Purification
- Purified by affinity chromatography, followed by gel filtration.
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- Application Notes
- Recombinant NFκB p50 is suitable for both gelshift and TransAM® assays. The standard curve for TransAM® NFκB p50 was generated using the range of 10 - 0.156 ng of protein. This protein is not recommended for Western blotting. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
- Restrictions
- For Research Use only
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- Concentration
- 100 ng/μL
- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- Nuclear Factor kappa B p50 (NFkB p50)
- Alternative Name
- NFkappaB p50
- Synonyms
- NF-KB1 Protein, NF-kappaB Protein, NF-kappaB1 Protein, p105 Protein, p50 Protein, p50/p105 Protein, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Protein, Nfkb1 Protein
- Background
- NFκB p50, is a subunit of the NFκB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel. p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains, they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IκB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IκB proteins. This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes.
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