Lysine (K)-Specific Demethylase 1A (KDM1A) protein (DYKDDDDK Tag)

Details for Product No. ABIN2669678
Protein Name
  • AOF2
  • BHC110
  • KDM1
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • DKFZp459N2450
  • RGD1562975
  • aof2
  • kdm1
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • KDM1A
  • lsd1
  • xLSD1
  • lysine demethylase 1A
  • lysine (K)-specific demethylase 1A
  • lysine (K)-specific demethylase 1a
  • KDM1A
  • Kdm1a
  • kdm1a
Origin
Human
7
1
1
1
Source
Baculovirus
3
2
2
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
DYKDDDDK Tag
Application
Enzyme Activity Assay (EAA), Screening Assay (ScA)
Options
Characteristics Recombinant LSD1 / KDM1A (accession number NP_055828) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 97.1 kDa.
Protein Name
Background LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.
Molecular Weight 97.1 kDa
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling
Application Notes Recombinant LSD1 / KDM1A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me2 and H3K4me1 demethylase. Histone Demethylase Assay Conditions: 50 mM TrisCl pH 8.25, 0.02 % Triton X100, 10 nM FAD, 5 nM Recombinant LSD1 / KDM1A protein, and 3.3 μM H3K4me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
Restrictions For Research Use only
Handling Advice Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Storage -80 °C
Storage Comment Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
Supplier Images
Image no. 1 for Lysine (K)-Specific Demethylase 1A (KDM1A) protein (DYKDDDDK Tag) (ABIN2669678) LSD1 / KDM1A activity assay. Recombinant LSD1 / KDM1A activity measured using a demet...
Image no. 2 for Lysine (K)-Specific Demethylase 1A (KDM1A) protein (DYKDDDDK Tag) (ABIN2669678) Recombinant LSD1 / KDM1A protein gel. LSD1 / KDM1A protein was run on a 10% SDS-PAGE ...
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