KDM5A Protein (DYKDDDDK Tag)

Catalog No. ABIN2669680

Product Details

Target: KDM5A (Lysine (K)-Specific Demethylase 5A (KDM5A))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus

Purification tag / Conjugate: This KDM5A protein is labelled with DYKDDDDK Tag.

Application: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Characteristics: Recombinant JARID1A / KDM5A (accession number NP_001036068.1) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 196.3 kDa.

Application Details

Application Notes: Recombinant JARID1A / KDM5A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Catalytic rate: >60 turnovers/ enzyme molecule/ hour. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JARID1A / KDM5A protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Target Details

Target: KDM5A (Lysine (K)-Specific Demethylase 5A (KDM5A))

Alternative Name: JARID1A / KDM5A (KDM5A Products)

Background: Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.

Molecular Weight: 196.3 kDa

Pathways: Chromatin Binding, Warburg Effect