KDM5B Protein (full length) (DYKDDDDK Tag)
KDM5B
Origin: Human
Host: Insect cells (Sf9)
Recombinant
EAA, ScA
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- Target See all KDM5B Proteins
- KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))
- Protein Type
- Recombinant
- Protein Characteristics
- full length
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This KDM5B protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant JARID1B / KDM5B (accession number NP_006609, full length) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 179.9 kDa.
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- Application Notes
- Recombinant JARID1B / KDM5B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Catalytic Rate: 20 turnovers/ enzyme molecule/ hour. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 50 nM Recombinant JARID1B / KDM5B protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))
- Alternative Name
- JARID1B / KDM5B (KDM5B Products)
- Background
- Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.
- Molecular Weight
- 179.9 kDa
- Pathways
- Warburg Effect
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