MLL3 Complex (AA 4740-4911) protein (GST tag)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
EAA, ScA
-
- Target
- MLL3 Complex
- Protein Type
- Recombinant
- Protein Characteristics
- AA 4740-4911
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- GST tag
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant MLL3 Complex (MLL3/WDR5/ASH2L/RBBP5/DPY30) was generated using a peptide corresponding to amino acids 4740-4911 (accession number NP_733751.2) of the MLL3 protein that was expressed in E. coli. Reconstituted MLL3-SET complex contains an N-terminal GST tag with a molecular weight of 46 kDa, human WDR5 (accession number NM_017588) that contains an N-terminal His-tag with a molecular weight of 35 kDa, human ASH2L (accession number NM_001105214) that contains an N-terminal His-tag with a molecular weight of 61 kDa, human RBBP5 (accession number NM_005057) that contains an N-terminal His-tag with a molecular weight of 60 kDa, and human DPY30 (accession number NM_032574) that contains an N-terminal His-tag with a molecular weight of 12 kDa. All Recombinant MLL3/WDR5/ASH2L/RBBP5/DPY30 Complex proteins were individually expressed in an E. coli expression system.
-
- Application Notes
- Recombinant MLL3 Complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: Histone methyltransferase with histone H3 lysine 4 methyltransferase activity. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 10 nM MLL3 Complex, 3.3 μM H3 (1-21) peptide at 2 hours at room temperature.
- Restrictions
- For Research Use only
-
- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
-
- Target
- MLL3 Complex
- Background
- The MLL3 Complex (MLL3/WDR5/ASH2L/RBBP5/DPY30), also known as the MLL3 WARD Complex is a histone methyltransferase that, like MLLs 1, 2 and 4, preferentially methylates the activating histone mark lysine 4 of histone H3 (H3K4). The MLL3 (also known as KTM2C) subunit contains the catalytic SET domain that is responsible for substrate recognition and enzymatic activity, along with the WDR5 and RBBP5 interaction motifs necessary for assembly of the WARD complex. The MLL3 WARD complex forms part of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. The chromatin remodeling complex SWI/SNF also interacts with ASCOM through binding of the MLL3 (or MLL4) SET domain with the INI1 subunit of SWI/SNF. Knockdown of MLLs 3 and 4 expression results in a reduction of HOXC10, an estrogen-induced protein that is overexpressed in breast cancer.
- Molecular Weight
- 12 kDa
-
You are here:
