LAP3 Protein (Myc-DYKDDDDK Tag)
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- Target See all LAP3 Proteins
- LAP3 (Cytosol Aminopeptidase (LAP3))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LAP3 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Cytosol aminopeptidase protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- LAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3, ) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 μl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours.
- Top Product
- Discover our top product LAP3 Protein
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Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (His tag)
custom-made LAP3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (His tag)custom-made LAP3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytosol Aminopeptidase (LAP3) (AA 252-368) protein (His-IF2DI Tag)LAP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (GST tag)LAP3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Cytosol Aminopeptidase (LAP3) (AA 1-519) protein (His tag)LAP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Cytosol Aminopeptidase (LAP3) (AA 25-64), (partial) protein (His-SUMO Tag)LAP3 Origin: Cow Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- > 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- LAP3 (Cytosol Aminopeptidase (LAP3))
- Alternative Name
- Cytosol Aminopeptidase (LAP3 Products)
- Background
- Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 56 kDa
- NCBI Accession
- NP_056991
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