LAP3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712411

Product Details

Target: LAP3 (Cytosol Aminopeptidase (LAP3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LAP3 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Cytosol aminopeptidase protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: LAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3, ) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 μl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: LAP3 (Cytosol Aminopeptidase (LAP3))

Alternative Name: Cytosol Aminopeptidase (LAP3 Products)

Synonyms: LAP Protein, LAPEP Protein, PEPS Protein, 2410015L10Rik Protein, AA410100 Protein, LAP-3 Protein, Lap Protein, Lapep Protein, Pep-7 Protein, Pep-S Protein, Pep7 Protein, Peps Protein, cytosol aminopeptidase Protein, leucine aminopeptidase 3 Protein, pepA Protein, Plabr_1567 Protein, Dester_0800 Protein, Weevi_0186 Protein, Marky_0570 Protein, Halhy_2623 Protein, FsymDg_3217 Protein, Flexsi_2186 Protein, Runsl_3980 Protein, lap3 Protein, LAP3 Protein, Lap3 Protein

Background: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 56 kDa

NCBI Accession: NP_056991