rho GTPase Activating Protein 22 (ARHGAP22) protein (His tag)

Details for Product No. ABIN2714877, Supplier: Log in to see
Protein Name
  • si:ch211-223a10.2
  • RhoGAP2
  • RhoGap22
  • B230341L19Rik
  • RHOGAP2
  • p68RacGAP
  • Rho GTPase activating protein 22
  • rho GTPase activating protein 22
  • ARHGAP22
  • arhgap22
  • CC1G_06014
  • Arhgap22
Origin
Human
2
1
Source
Escherichia coli (E. coli)
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This ARHGAP22 protein is labelled with His tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human ARHGAP22 (full length, N-term HIS tag) protein expressed in E. coli.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Background This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 76.6 kDa
NCBI Accession NP_067049
Research Area Signaling
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the N-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for rho GTPase Activating Protein 22 (ARHGAP22) protein (His tag) (ABIN2714877) Validation with Western Blot
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