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YWHAE Protein (Transcript Variant 1) (His tag)

YWHAE Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2715554
  • Target See all YWHAE Proteins
    YWHAE (14-3-3 epsilon (YWHAE))
    Protein Type
    Recombinant
    Protein Characteristics
    Transcript Variant 1
    Origin
    • 12
    • 3
    • 1
    • 1
    • 1
    Human
    Source
    • 9
    • 3
    • 3
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This YWHAE protein is labelled with His tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human 14-3-3 protein epsilon (transcript variant 1) protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    Purity
    > 95 % as determined by SDS-PAGE and Coomassie blue staining
    Endotoxin Level
    < 0.1 EU per μg protein as determined by LAL test
    Top Product
    Discover our top product YWHAE Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Buffer
    Lyophilized from a 0.2 μM filtered solution of 20 mM Phosphate buffer, 150 mM NaCl, pH 7.2. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    YWHAE (14-3-3 epsilon (YWHAE))
    Alternative Name
    14-3-3 Protein epsilon (YWHAE Products)
    Background
    This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.
    Molecular Weight
    56 kDa
    NCBI Accession
    NP_006752
    Pathways
    Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
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