PHD1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all PHD1 (EGLN2) Proteins
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PHD1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human EGLN2 / PHD1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product EGLN2 Protein
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Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-407) protein (GST tag)
EGLN2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) protein (His tag)EGLN2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 283-407), (partial) protein (His tag)EGLN2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer." in: Nature cell biology, Vol. 18, Issue 2, pp. 213-24, (2016) (PubMed).
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: "The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer." in: Nature cell biology, Vol. 18, Issue 2, pp. 213-24, (2016) (PubMed).
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- Target
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
- Alternative Name
- Egln2,phd1 (EGLN2 Products)
- Background
- The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
- Molecular Weight
- 43.5 kDa
- NCBI Accession
- NP_444274
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis
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