GRAP Protein (Myc-DYKDDDDK Tag)
Quick Overview for GRAP Protein (Myc-DYKDDDDK Tag) (ABIN2722222)
Target
See all GRAP ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This GRAP protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human GRAP protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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GRB2-Related Adaptor Protein (GRAP) (AA 1-217) protein (His tag)
custom-made GRAP Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
GRB2-Related Adaptor Protein (GRAP) (AA 1-217) protein (His tag)custom-made GRAP Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
GRB2-Related Adaptor Protein (GRAP) (AA 1-217) protein (GST tag)GRAP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- GRAP (GRB2-Related Adaptor Protein (GRAP))
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Alternative Name
- Grap
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Background
- This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
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Molecular Weight
- 25.2 kDa
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NCBI Accession
- NP_006604
Target
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