GRAP Protein (Myc-DYKDDDDK Tag)
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- Target See all GRAP Proteins
- GRAP (GRB2-Related Adaptor Protein (GRAP))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GRAP protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human GRAP protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product GRAP Protein
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GRB2-Related Adaptor Protein (GRAP) (AA 1-217) protein (GST tag)
GRAP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
GRB2-Related Adaptor Protein (GRAP) (AA 1-217) protein (Strep Tag)Crystallography grade GRAP Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
GRB2-Related Adaptor Protein (GRAP) (AA 1-217) protein (Strep Tag)Crystallography grade GRAP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GRAP (GRB2-Related Adaptor Protein (GRAP))
- Alternative Name
- Grap (GRAP Products)
- Synonyms
- 8430435N19Rik Protein, BB233594 Protein, GRAP Protein, grap Protein, zgc:110202 Protein, MGC109892 Protein, si:dkeyp-9d4.1 Protein, Grap Protein, GRB2-related adaptor protein Protein, GRB2-related adapter protein Protein, GRB2-related adaptor protein a Protein, GRB2-related adaptor protein b Protein, GRAP Protein, Grap Protein, LOC489534 Protein, grapa Protein, grapb Protein, LOC100727005 Protein
- Background
- This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
- Molecular Weight
- 25.2 kDa
- NCBI Accession
- NP_006604
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