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Osteopontin Protein (Transcript Variant 2) (His tag)

SPP1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2728021
  • Target See all Osteopontin (SPP1) Proteins
    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
    Protein Type
    Recombinant
    Protein Characteristics
    Transcript Variant 2
    Origin
    • 21
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 20
    • 12
    • 8
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This Osteopontin protein is labelled with His tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human Osteopontin / SPP1 (transcript variant 2) protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 95 % as determined by SDS-PAGE and Coomassie blue staining
    Endotoxin Level
    < 0.1 EU per μg protein as determined by LAL test
    Top Product
    Discover our top product SPP1 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the N-terminal.

    Restrictions
    For Research Use only
  • Buffer
    Lyophilized from a 0.2 μM filtered solution of 20 mM Phosphate buffer, 150 mM NaCl, pH 7.2. Stable for at least 6 months from date of receipt under proper storage and handling conditions.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
    Alternative Name
    Osteopontin,spp1 (SPP1 Products)
    Background
    The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
    Molecular Weight
    34.7 kDa
    NCBI Accession
    NP_000573
    Pathways
    Regulation of Cell Size
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