phosphodiesterase 4C, CAMP-Specific (PDE4C) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2728516
Protein Name
Protein Characteristics
Transcript Variant 2
1
1
1
Origin
Human
3
2
1
Source
HEK-293 Cells
2
1
1
Protein Type
Recombinant
Purification tag / Conjugate
Myc-DYKDDDDK Tag
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human PDE4C (transcript variant 2) protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011].
Molecular Weight 67.6 kDa
NCBI Accession NP_001092289
Pathways Negative Regulation of Hormone Secretion, cAMP Metabolic Process
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Images
Image no. 1 for phosphodiesterase 4C, CAMP-Specific (PDE4C) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag) (ABIN2728516) Validation with Western Blot