RAD18 Protein (Myc-DYKDDDDK Tag)
RAD18
Origin: Human
Host: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
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(1 reference)-
- Target See all RAD18 Proteins
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RAD18 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human RAD18 / RNF73 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product RAD18 Protein
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E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) protein (GST tag)
RAD18 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495), (full length) protein (His-SUMO Tag)RAD18 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "USP7 is essential for maintaining Rad18 stability and DNA damage tolerance." in: Oncogene, Vol. 35, Issue 8, pp. 965-76, (2016) (PubMed).
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: "USP7 is essential for maintaining Rad18 stability and DNA damage tolerance." in: Oncogene, Vol. 35, Issue 8, pp. 965-76, (2016) (PubMed).
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- Target
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
- Alternative Name
- Rad18,rnf73 (RAD18 Products)
- Background
- The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
- Molecular Weight
- 56 kDa
- NCBI Accession
- NP_064550
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