RAP2B, Member of RAS Oncogene Family (RAP2B) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2730479
Protein Name
Origin
Human
5
1
1
Source
HEK-293 Cells
4
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This RAP2B protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human RAP2B protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50 % amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated.
Molecular Weight 20.3 kDa
NCBI Accession NP_002877
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Image no. 1 for RAP2B, Member of RAS Oncogene Family (RAP2B) protein (Myc-DYKDDDDK Tag) (ABIN2730479) Validation with Western Blot