Reticulocalbin 1, EF-Hand Calcium Binding Domain (RCN1) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2730743, Supplier: Log in to see
Protein Name
  • Rcn
  • PIG20
  • RCAL
  • RCN
  • reticulocalbin
  • reticulocalbin 1
  • Smp_005230.3
  • Rcn1
  • RCN1
Origin
Human
6
1
1
Source
HEK-293 Cells
5
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This RCN1 protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human Reticulocalbin-1 / RCN1 protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Background Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009].
Molecular Weight 35.8 kDa
NCBI Accession NP_002892
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for Reticulocalbin 1, EF-Hand Calcium Binding Domain (RCN1) protein (Myc-DYKDDDDK Tag) (ABIN2730743) Validation with Western Blot
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