ube3a Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
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- Target See all ube3a Proteins
- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 3
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ube3a protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human UBE3A (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ube3a Protein
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Ubiquitin Protein Ligase E3A (ube3a) (AA 51-150) protein (GST tag)
ube3a Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin Protein Ligase E3A (ube3a) (AA 1-875) protein (GST tag)ube3a Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin Protein Ligase E3A (ube3a) (AA 1-875) protein (His tag)custom-made ube3a Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin Protein Ligase E3A (ube3a) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)ube3a Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- ube3a (Ubiquitin Protein Ligase E3A (ube3a))
- Alternative Name
- Ube3a (ube3a Products)
- Background
- This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
- Molecular Weight
- 99.9 kDa
- NCBI Accession
- NP_570854
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway
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