Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2735000
Protein Name
Protein Characteristics
Transcript Variant 2
3
1
1
1
Origin
Human
5
1
Source
HEK-293 Cells
3
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This UHRF1 protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human UHRF1 (transcript variant 2) protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
Molecular Weight 90.9 kDa
NCBI Accession NP_037414
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Image no. 1 for Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag) (ABIN2735000) Validation with Western Blot