WIT1 Protein (Myc-DYKDDDDK Tag)
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- Target See all WIT1 (WT1-AS) products
- WIT1 (WT1-AS) (WT1 Antisense RNA (WT1-AS))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This WIT1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Wilms tumor upstream neighbor 1 (WIT1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- WIT1 (WT1-AS) (WT1 Antisense RNA (WT1-AS))
- Alternative Name
- Wilms Tumor Upstream Neighbor 1 (Wit1) (WT1-AS Products)
- Background
- This gene is located upstream of the Wilms tumor 1 (WT1) gene these two genes are bi-directionally transcribed from the same promoter region. This gene is imprinted in kidney, with preferential expression from the paternal allele. Imprinting defects at chromosome 11p13 may contribute to tumorigenesis.
- Molecular Weight
- 9.9 kDa
- NCBI Accession
- NP_056939
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