Flagellin Protein (FliC) (AA 1-495) (His tag)

Catalog No. ABIN2745676

Product Details

Target: Flagellin (FliC)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-495

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Flagellin protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purpose: Flagellin (rec.)

Specificity: Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).

Cross-Reactivity: Human, Mouse

Characteristics: The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) is fused at the N-terminus to a His-tag.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (rec.) at concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5μg/ml on bone-marrow macrophages in the presence of streptolysin O (SLO, 25μg/ml), a protein that allows delivery of exogenous molecules into the cytosol of living cells.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Flagellin (FliC)

Alternative Name: Flagellin (FliC Products)

Background:

FliC

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

Molecular Weight: ~54kDa (SDS-PAGE)

UniProt: P06179

Pathways: Inflammasome